Molecular epidemiology and molecular characterization of respiratory syncytial viruses at a tertiary care university hospital in Catalonia (Spain) during the 2013-2014 season.
Identifieur interne : 000176 ( Main/Exploration ); précédent : 000175; suivant : 000177Molecular epidemiology and molecular characterization of respiratory syncytial viruses at a tertiary care university hospital in Catalonia (Spain) during the 2013-2014 season.
Auteurs : Laura Gimferrer [Espagne] ; Magda Campins [Espagne] ; María Gema Codina [Espagne] ; María Del Carmen Martín [Espagne] ; Francisco Fuentes [Espagne] ; Juliana Esperalba [Espagne] ; Andreu Bruguera [Espagne] ; Luz María Vilca [Espagne] ; Lluís Armadans [Espagne] ; Tomàs Pumarola [Espagne] ; Andrés Ant N [Espagne]Source :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [ 1873-5967 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Analyse de séquence d'ADN (MeSH), Données de séquences moléculaires (MeSH), Enfant d'âge préscolaire (MeSH), Espagne (épidémiologie), Femelle (MeSH), Génotype (MeSH), Humains (MeSH), Hôpitaux universitaires (MeSH), Infections à virus respiratoire syncytial (virologie), Infections à virus respiratoire syncytial (épidémiologie), Mâle (MeSH), Nourrisson (MeSH), Phylogenèse (MeSH), Protéines de fusion virale (génétique), Protéines de l'enveloppe virale (génétique), Similitude de séquences (MeSH), Soins de santé tertiaires (MeSH), Sujet âgé (MeSH), Sujet âgé de 80 ans ou plus (MeSH), Virus respiratoire syncytial humain (classification), Virus respiratoire syncytial humain (génétique), Virus respiratoire syncytial humain (isolement et purification), Épidémiologie moléculaire (MeSH).
- MESH :
- génétique : ARN viral, Protéines de fusion virale, Protéines de l'enveloppe virale, Virus respiratoire syncytial humain.
- isolement et purification : Virus respiratoire syncytial humain.
- virologie : Infections à virus respiratoire syncytial.
- épidémiologie : Espagne, Infections à virus respiratoire syncytial.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Enfant d'âge préscolaire, Femelle, Génotype, Humains, Hôpitaux universitaires, Mâle, Nourrisson, Phylogenèse, Similitude de séquences, Soins de santé tertiaires, Sujet âgé, Sujet âgé de 80 ans ou plus, Épidémiologie moléculaire.
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- Aged (MeSH), Aged, 80 and over (MeSH), Child, Preschool (MeSH), Female (MeSH), Genotype (MeSH), Hospitals, University (MeSH), Humans (MeSH), Infant (MeSH), Male (MeSH), Molecular Epidemiology (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), RNA, Viral (genetics), Respiratory Syncytial Virus Infections (epidemiology), Respiratory Syncytial Virus Infections (virology), Respiratory Syncytial Virus, Human (classification), Respiratory Syncytial Virus, Human (genetics), Respiratory Syncytial Virus, Human (isolation & purification), Sequence Analysis, DNA (MeSH), Sequence Homology (MeSH), Spain (epidemiology), Tertiary Healthcare (MeSH), Viral Envelope Proteins (genetics), Viral Fusion Proteins (genetics).
- MESH :
- chemical , genetics : RNA, Viral, Viral Envelope Proteins, Viral Fusion Proteins.
- geographic , epidemiology : Spain.
- classification : Respiratory Syncytial Virus, Human.
- epidemiology : Respiratory Syncytial Virus Infections.
- genetics : Respiratory Syncytial Virus, Human.
- isolation & purification : Respiratory Syncytial Virus, Human.
- virology : Respiratory Syncytial Virus Infections.
- Aged, Aged, 80 and over, Child, Preschool, Female, Genotype, Hospitals, University, Humans, Infant, Male, Molecular Epidemiology, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Sequence Homology, Tertiary Healthcare.
Abstract
BACKGROUND
Human respiratory syncytial virus (HRSV) is the main cause of lower respiratory tract infections among infants and young children.
OBJECTIVES
The molecular epidemiology and characterization of HRSV strains detected at a Spanish tertiary hospital during the 2013-2014 season is reported.
STUDY DESIGN
Phylogenetic analysis and molecular characterization of HRSV laboratory-confirmed respiratory samples were performed, based on coding sequences of G and F proteins.
RESULTS
HRSV infection was laboratory-confirmed in respiratory samples from 320 patients of which 223 (70%) were less than 2 years of age and none undergoing Palivizumab treatment. HRSV was detected at varying levels throughout the season with a maximum rate in the week 52/2013, right before the beginning of the influenza epidemic. Whilst both HRSV groups were found co-circulating, HRSV-B group clearly predominated. The phylogenetic analyses from 139 HVR-2 sequences revealed that most characterized strains belonged to ON1 and BA9 genotypes. Three different phylogenetic subgroups could be distinguished within these genotypes. In addition, three strains (out of the 52 randomly selected) were carrying amino acid substitutions in the epitope A of the F protein, one of them previously related to Palivizumab resistance.
CONCLUSIONS
The results of the present study highlight the importance of a continuous HRSV surveillance to monitor not only the introduction of new genotypes on circulation but also the emergence of viral variants with new genetic characteristics that can affect the antigenicity features and the susceptibility to the only current prophylaxis treatment and also for the future development of HRSV vaccines.
DOI: 10.1016/j.jcv.2015.02.018
PubMed: 25866332
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Bruguera, Andreu" sort="Bruguera, Andreu" uniqKey="Bruguera A" first="Andreu" last="Bruguera">Andreu Bruguera</name><affiliation wicri:level="3"><nlm:affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement 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Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Pumarola, Tomas" sort="Pumarola, Tomas" uniqKey="Pumarola T" first="Tomàs" last="Pumarola">Tomàs Pumarola</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, 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sortKey="Campins, Magda" sort="Campins, Magda" uniqKey="Campins M" first="Magda" last="Campins">Magda Campins</name><affiliation wicri:level="3"><nlm:affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Codina, Maria Gema" sort="Codina, Maria Gema" uniqKey="Codina M" first="María Gema" last="Codina">María Gema Codina</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Martin, Maria Del Carmen" sort="Martin, Maria Del Carmen" uniqKey="Martin M" first="María Del Carmen" last="Martín">María Del Carmen Martín</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Fuentes, Francisco" sort="Fuentes, Francisco" uniqKey="Fuentes F" first="Francisco" last="Fuentes">Francisco Fuentes</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Esperalba, Juliana" sort="Esperalba, Juliana" uniqKey="Esperalba J" first="Juliana" last="Esperalba">Juliana Esperalba</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Bruguera, Andreu" sort="Bruguera, Andreu" uniqKey="Bruguera A" first="Andreu" last="Bruguera">Andreu Bruguera</name><affiliation wicri:level="3"><nlm:affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Vilca, Luz Maria" sort="Vilca, Luz Maria" uniqKey="Vilca L" first="Luz María" last="Vilca">Luz María Vilca</name><affiliation wicri:level="3"><nlm:affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Armadans, Lluis" sort="Armadans, Lluis" uniqKey="Armadans L" first="Lluís" last="Armadans">Lluís Armadans</name><affiliation wicri:level="3"><nlm:affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Pumarola, Tomas" sort="Pumarola, Tomas" uniqKey="Pumarola T" first="Tomàs" last="Pumarola">Tomàs Pumarola</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Ant N, Andres" sort="Ant N, Andres" uniqKey="Ant N A" first="Andrés" last="Ant N">Andrés Ant N</name><affiliation wicri:level="3"><nlm:affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain. Electronic address: aanton@vhebron.net.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author></analytic><series><title level="j">Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</title><idno type="eISSN">1873-5967</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged (MeSH)</term><term>Aged, 80 and over (MeSH)</term><term>Child, Preschool (MeSH)</term><term>Female (MeSH)</term><term>Genotype (MeSH)</term><term>Hospitals, University (MeSH)</term><term>Humans (MeSH)</term><term>Infant (MeSH)</term><term>Male (MeSH)</term><term>Molecular Epidemiology (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>RNA, Viral (genetics)</term><term>Respiratory Syncytial Virus Infections (epidemiology)</term><term>Respiratory Syncytial Virus Infections (virology)</term><term>Respiratory Syncytial Virus, Human (classification)</term><term>Respiratory Syncytial Virus, Human (genetics)</term><term>Respiratory Syncytial Virus, Human (isolation & purification)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sequence Homology (MeSH)</term><term>Spain (epidemiology)</term><term>Tertiary Healthcare (MeSH)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Fusion Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Enfant d'âge préscolaire (MeSH)</term><term>Espagne (épidémiologie)</term><term>Femelle (MeSH)</term><term>Génotype (MeSH)</term><term>Humains (MeSH)</term><term>Hôpitaux universitaires (MeSH)</term><term>Infections à virus respiratoire syncytial (virologie)</term><term>Infections à virus respiratoire syncytial (épidémiologie)</term><term>Mâle (MeSH)</term><term>Nourrisson (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Protéines de fusion virale (génétique)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Similitude de séquences (MeSH)</term><term>Soins de santé tertiaires (MeSH)</term><term>Sujet âgé (MeSH)</term><term>Sujet âgé de 80 ans ou plus (MeSH)</term><term>Virus respiratoire syncytial humain (classification)</term><term>Virus respiratoire syncytial humain (génétique)</term><term>Virus respiratoire syncytial humain (isolement et purification)</term><term>Épidémiologie moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Spain</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Respiratory Syncytial Virus, Human</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Respiratory Syncytial Virus Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Respiratory Syncytial Virus, Human</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Virus respiratoire syncytial humain</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Respiratory Syncytial Virus, Human</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus respiratoire syncytial humain</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à virus respiratoire syncytial</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Respiratory Syncytial Virus Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Espagne</term><term>Infections à virus respiratoire syncytial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Child, Preschool</term><term>Female</term><term>Genotype</term><term>Hospitals, University</term><term>Humans</term><term>Infant</term><term>Male</term><term>Molecular Epidemiology</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Sequence Homology</term><term>Tertiary Healthcare</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Génotype</term><term>Humains</term><term>Hôpitaux universitaires</term><term>Mâle</term><term>Nourrisson</term><term>Phylogenèse</term><term>Similitude de séquences</term><term>Soins de santé tertiaires</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Épidémiologie moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Human respiratory syncytial virus (HRSV) is the main cause of lower respiratory tract infections among infants and young children.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b></p><p>The molecular epidemiology and characterization of HRSV strains detected at a Spanish tertiary hospital during the 2013-2014 season is reported.</p></div><div type="abstract" xml:lang="en"><p><b>STUDY DESIGN</b></p><p>Phylogenetic analysis and molecular characterization of HRSV laboratory-confirmed respiratory samples were performed, based on coding sequences of G and F proteins.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>HRSV infection was laboratory-confirmed in respiratory samples from 320 patients of which 223 (70%) were less than 2 years of age and none undergoing Palivizumab treatment. HRSV was detected at varying levels throughout the season with a maximum rate in the week 52/2013, right before the beginning of the influenza epidemic. Whilst both HRSV groups were found co-circulating, HRSV-B group clearly predominated. The phylogenetic analyses from 139 HVR-2 sequences revealed that most characterized strains belonged to ON1 and BA9 genotypes. Three different phylogenetic subgroups could be distinguished within these genotypes. In addition, three strains (out of the 52 randomly selected) were carrying amino acid substitutions in the epitope A of the F protein, one of them previously related to Palivizumab resistance.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The results of the present study highlight the importance of a continuous HRSV surveillance to monitor not only the introduction of new genotypes on circulation but also the emergence of viral variants with new genetic characteristics that can affect the antigenicity features and the susceptibility to the only current prophylaxis treatment and also for the future development of HRSV vaccines.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25866332</PMID><DateCompleted><Year>2015</Year><Month>12</Month><Day>22</Day></DateCompleted><DateRevised><Year>2015</Year><Month>04</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-5967</ISSN><JournalIssue CitedMedium="Internet"><Volume>66</Volume><PubDate><Year>2015</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology</Title><ISOAbbreviation>J. Clin. Virol.</ISOAbbreviation></Journal><ArticleTitle>Molecular epidemiology and molecular characterization of respiratory syncytial viruses at a tertiary care university hospital in Catalonia (Spain) during the 2013-2014 season.</ArticleTitle><Pagination><MedlinePgn>27-32</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jcv.2015.02.018</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1386-6532(15)00066-9</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Human respiratory syncytial virus (HRSV) is the main cause of lower respiratory tract infections among infants and young children.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">The molecular epidemiology and characterization of HRSV strains detected at a Spanish tertiary hospital during the 2013-2014 season is reported.</AbstractText><AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">Phylogenetic analysis and molecular characterization of HRSV laboratory-confirmed respiratory samples were performed, based on coding sequences of G and F proteins.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">HRSV infection was laboratory-confirmed in respiratory samples from 320 patients of which 223 (70%) were less than 2 years of age and none undergoing Palivizumab treatment. HRSV was detected at varying levels throughout the season with a maximum rate in the week 52/2013, right before the beginning of the influenza epidemic. Whilst both HRSV groups were found co-circulating, HRSV-B group clearly predominated. The phylogenetic analyses from 139 HVR-2 sequences revealed that most characterized strains belonged to ON1 and BA9 genotypes. Three different phylogenetic subgroups could be distinguished within these genotypes. In addition, three strains (out of the 52 randomly selected) were carrying amino acid substitutions in the epitope A of the F protein, one of them previously related to Palivizumab resistance.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The results of the present study highlight the importance of a continuous HRSV surveillance to monitor not only the introduction of new genotypes on circulation but also the emergence of viral variants with new genetic characteristics that can affect the antigenicity features and the susceptibility to the only current prophylaxis treatment and also for the future development of HRSV vaccines.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gimferrer</LastName><ForeName>Laura</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Campins</LastName><ForeName>Magda</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Codina</LastName><ForeName>María Gema</ForeName><Initials>MG</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martín</LastName><ForeName>María Del Carmen</ForeName><Initials>Mdel C</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fuentes</LastName><ForeName>Francisco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Esperalba</LastName><ForeName>Juliana</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bruguera</LastName><ForeName>Andreu</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vilca</LastName><ForeName>Luz María</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Armadans</LastName><ForeName>Lluís</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Preventive Medicine and Epidemiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pumarola</LastName><ForeName>Tomàs</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Antón</LastName><ForeName>Andrés</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Virology Unit, Microbiology Department, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain. 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PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25866332</ArticleId><ArticleId IdType="pii">S1386-6532(15)00066-9</ArticleId><ArticleId IdType="doi">10.1016/j.jcv.2015.02.018</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Catalogne</li></region><settlement><li>Barcelone</li></settlement></list><tree><country name="Espagne"><region name="Catalogne"><name sortKey="Gimferrer, Laura" sort="Gimferrer, Laura" uniqKey="Gimferrer L" first="Laura" last="Gimferrer">Laura Gimferrer</name></region><name sortKey="Ant N, Andres" sort="Ant N, Andres" uniqKey="Ant N A" first="Andrés" last="Ant N">Andrés Ant N</name><name sortKey="Armadans, Lluis" sort="Armadans, Lluis" uniqKey="Armadans L" first="Lluís" last="Armadans">Lluís Armadans</name><name sortKey="Bruguera, Andreu" sort="Bruguera, Andreu" uniqKey="Bruguera A" first="Andreu" last="Bruguera">Andreu Bruguera</name><name sortKey="Campins, Magda" sort="Campins, Magda" uniqKey="Campins M" first="Magda" last="Campins">Magda Campins</name><name sortKey="Codina, Maria Gema" sort="Codina, Maria Gema" uniqKey="Codina M" first="María Gema" last="Codina">María Gema Codina</name><name sortKey="Esperalba, Juliana" sort="Esperalba, Juliana" uniqKey="Esperalba J" first="Juliana" last="Esperalba">Juliana Esperalba</name><name sortKey="Fuentes, Francisco" sort="Fuentes, Francisco" uniqKey="Fuentes F" first="Francisco" last="Fuentes">Francisco Fuentes</name><name sortKey="Martin, Maria Del Carmen" sort="Martin, Maria Del Carmen" uniqKey="Martin M" first="María Del Carmen" last="Martín">María Del Carmen Martín</name><name sortKey="Pumarola, Tomas" sort="Pumarola, Tomas" uniqKey="Pumarola T" first="Tomàs" last="Pumarola">Tomàs Pumarola</name><name sortKey="Vilca, Luz Maria" sort="Vilca, Luz Maria" uniqKey="Vilca L" first="Luz María" last="Vilca">Luz María Vilca</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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